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Journal: American Journal of Physiology - Cell Physiology
Article Title: Thermal injury initiates pervasive fibrogenesis in skeletal muscle
doi: 10.1152/ajpcell.00337.2019
Figure Lengend Snippet: Collagen deposition is elevated in skeletal muscle after severe burn injury and not resolved by 21 days postburn. A: total collagen content in spinotrapezius as measured by hydroxyproline content in muscle pellet. B: pyridinium crosslinks relative to total collagen content measured by hydroxyproline in spintrapezius muscle pellet. HOP, hydroxyproline. C: collagen 1 content relative to area of spinotrapezius cross section. D: collagen turnover measured by collagen hybridizing peptide (CHP) content relative to area of spinotrapezius cross section. E: collagen 3 content relative to area of spinotrapezius cross section. F: collagen 4 content relative to area of spinotrapezius cross section. G: representative image of collagen 4 content in spinotrapezius cross section after burn injury. H: representative image of collagen 3 content in spinotrapezius cross section after burn injury. I: representative images of collagen 1 and CHP content in spinotrapezius cross section after burn injury. Data are presented as means ± SE. One-way ANOVA with Tukey’s post hoc test. *P < 0.05 compared with sham. #P = 0.051 compared with sham; n = 4–5 mice/group.
Article Snippet: Sections were then blocked in 1% blocking reagent included in a commercially available TSA kit (Life Technologies/Thermo Fisher Scientific) for 1 h, followed by overnight incubation in
Techniques:
Journal: American Journal of Physiology - Cell Physiology
Article Title: Thermal injury initiates pervasive fibrogenesis in skeletal muscle
doi: 10.1152/ajpcell.00337.2019
Figure Lengend Snippet: Fibrogenic cell abundance is related to skeletal muscle collagen content after severe burn injury. Correlation of fibrogenic adipogenic progenitor cell (FAP) abundance relative to myofiber number with total collagen in the muscle pellet as measured by hydroxyproline content of spinotrapezius (A); collagen 1 content relative to area of spinotrapezius cross section (B); collagen 3 content relative to area of spinotrapezius cross section (C); and collagen 4 content relative to area of spinotrapezius cross section (D). Pearson correlation. n = 4–5 mice/group.
Article Snippet: Sections were then blocked in 1% blocking reagent included in a commercially available TSA kit (Life Technologies/Thermo Fisher Scientific) for 1 h, followed by overnight incubation in
Techniques: